An Inter‐Subspecies Cloned Buffalo (Bubalus bubalis) Obtained by Transferring of Cryopreserved Embryos via Somatic Cell Nuclear Transfer     

BZ Yang  CY Yang  RC Li  GS Qin  XF Zhang  CY Pang  MT Chen  FX Huang  Z Li  HY Zheng  YJ Huang  XW Liang 《Reproduction in domestic animals》2010,45(5):e21-e25

The aim of this study was to explore the feasibility of cryopreservation of inter‐subspecies cloned embryos in buffalo. In our experiment, river buffalo ear fibroblast nucleus was fused into swamp buffalo oocyte cytoplasm. The blastocyst formation rate for nuclear transfer of freshly thawed cells was not different from those of growing cells, confluent or serum‐starved cells. A total of 122 cloned blastocysts derived from cryopreserved fibroblasts were cryopreserved and thawed, 37 were survived, the cryosurvival rate was 30.3%. The survived blastocysts were transferred into 15 recipient buffalos. Five of the recipients established pregnancy, but four of them aborted on day 53, 59, 145 and 179 of gestation respectively. One cross‐bred buffalo (Murrah × Swamp buffalo (2n = 49) received three embryos delivered a 40.5 kg female calf by natural delivery on day 320 of gestation. Up to now (13‐month old), the cloned calf has been growing well with no abnormity observed. These results demonstrated that cryopreservation of inter‐subspecies cloned embryos is feasible to produce buffalo offspring.  相似文献   

Demodectic mange in threatened southern sea otters (Enhydra lutris nereis)     

Nadia Javeed  Janet Foley  Arturo Oliver-Guimera  Verena K. Affolter  Michael K. Keel  Angelina Reed  Risa Pesapane  Pádraig J. Duignan  Michael Murray  MT Tinker  Melissa A. Miller 《Veterinary dermatology》2021,32(3):211-e55

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Onset of Spermatogenesis in Meishan and Large White Male Piglets     

MT Hochereau-de Reviers  C Perreau  V Rahal-Perola  D Royere 《Reproduction in domestic animals》1999,34(3-4):213-217

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Pleistocene extinction of genyornis newtoni: human impact on australian megafauna     

GH Miller  JW Magee  BJ Johnson  ML Fogel  NA Spooner  MT McCulloch  LK Ayliffe 《Science (New York, N.Y.)》1999,283(5399):205-208

More than 85 percent of Australian terrestrial genera with a body mass exceeding 44 kilograms became extinct in the Late Pleistocene. Although most were marsupials, the list includes the large, flightless mihirung Genyornis newtoni. More than 700 dates onGenyornis eggshells from three different climate regions document the continuous presence of Genyornis from more than 100,000 years ago until their sudden disappearance 50,000 years ago, about the same time that humans arrived in Australia. Simultaneous extinction of Genyornis at all sites during an interval of modest climate change implies that human impact, not climate, was responsible.  相似文献   

Killer whale predation on sea otters linking oceanic and nearshore ecosystems   总被引：2，自引：0，他引：2  

JA Estes  MT Tinker  TM Williams  DF Doak 《Science (New York, N.Y.)》1998,282(5388):473-476

After nearly a century of recovery from overhunting, sea otter populations are in abrupt decline over large areas of western Alaska. Increased killer whale predation is the likely cause of these declines. Elevated sea urchin density and the consequent deforestation of kelp beds in the nearshore community demonstrate that the otter's keystone role has been reduced or eliminated. This chain of interactions was probably initiated by anthropogenic changes in the offshore oceanic ecosystem.  相似文献